|
|
| Huvet, Arnaud; Boudry, Pierre; Ohresser, M; Delsert, Claude; Bonhomme, F. |
| Source/description : Genomic DNA was extracted from a whole Pacific oyster (C. gigas) after grinding in liquid nitrogen. Purified DNA was then digested by a mix of 3 restriction enzymes (AluI, RsaI, HaeIII). DNA fragments ranging from 250 to 500 bp were size-selected by agarose gel electrophoresis and ligated into the dephosphorylated blunt-ended SmaI-linearised pBKS2 plasmid (Stratagene Cloning System, CA). White colonies (11,500) were screened by hybridisation with double-stranded DNA probes containing dinucleotide repeats. Poly(dAdC), poly(dAdG), poly(dAdT) (Pharmacia Biotech, Sweden) were mixed at equimolar ratio and radiolabelled by [-32P] alpha dATP and dCTP using a random priming labelling kit (Life Technologies, Germany). Filter... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Oyster; Microsatellites. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-703.pdf |
| |
